High-resolution frequency-domain second-harmonic optical coherence tomography.
نویسندگان
چکیده
We used continuum generated in an 8.5 cm long fiber by a femtosecond Yb fiber laser to improve threefold the axial resolution of frequency domain second-harmonic optical coherence tomography (SH-OCT) to 12 microm. The acquisition time was shortened by more than 2 orders of magnitude compared to the time-domain SH-OCT. The system was applied to image biological tissue of fish scales, pig leg tendon, and rabbit eye sclera. Highly organized collagen fibrils can be visualized in the recorded images. Polarization dependence on the SH has been used to obtain polarization resolved images.
منابع مشابه
High-resolution second-harmonic optical coherence tomography of collagen in rat-tail tendon
A high-resolution second-harmonic optical coherence tomography sSH-OCTd system is demonstrated using a spectrum broadened femtosecond Ti:sapphire laser. An axial resolution of 4.2 mm at the second-harmonic wave center wavelength of 400 nm has been achieved. Because the SH-OCT system uses the second-harmonic generation signals that strongly depend on the orientation, polarization, and local symm...
متن کاملPolarization-resolved second-harmonic-generation optical coherence tomography in collagen.
We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We d...
متن کاملDevelopment of an Advanced Optical Coherence Tomography System for Radiation Dosimetry
Introduction: According to the literature, optical coherence tomography (OCT) can be used measure radiation absorbed dose. This study was carried out to design a computed tomography system for the calculation of absorbed dose and optimization of dose delivery in radiotherapy using gel dosimeters. Material and Methods: An advanced charge-coupled device based OCT system was developed in laborator...
متن کاملExperimental Visualization of Labyrinthine Structure with Optical Coherence Tomography
Introduction:Visualization of inner ear structures is a valuable strategy for researchers and clinicians working on hearing pathologies. Optical coherence tomography (OCT) is a high-resolution imaging technology which may be used for the visualization of tissues. In this experimental study we aimed to evaluate inner ear anatomy in well-prepared human labyrinthine bones.Materials and Methods:Thr...
متن کاملDemonstration of complex-conjugate-resolved harmonic Fourier-domain optical coherence tomography imaging of biological samples.
Complex-conjugate-resolved Fourier-domain optical coherence tomography, where the quadrature components of the interferogram are obtained by simultaneous acquisition of the first and second harmonics of the phase-modulated interferogram, is applied to multisurface test targets and biological samples. The method provides efficient suppression of the complex-conjugate, dc, and autocorrelation art...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Applied optics
دوره 46 10 شماره
صفحات -
تاریخ انتشار 2007